HI SIR. FOR LAB REPORT I FOUNF THE URL

HI SIR. FOR LAB REPORT I FOUNF THE URL
2020 Autumn Special Notes for Laboratory BIOL S356F 1. Time points for PCR Session Time Collect samples for PCR Return PCR products 10 a.m. 12:30 p.m. 12:30 p.m. Beginning of next session 10 a.m. 12:30 p.m. 12:30 p.m. Beginning of next session 10 a.m. 12:30 p.m. 12:30 p.m. Beginning of next session For lab sessions from 9 a.m. to 1 p.m.: For lab sessions from 2 p.m. to 6 p.m.: Session Time Collect samples for PCR Return PCR products 3 p.m. 5:30 p.m. 5:30 p.m. Beginning of next session 3 p.m. 5:30 p.m. 5:30 p.m. Beginning of next session 3 p.m. 5:30 p.m. 5:30 p.m. Beginning of next session 2. Limited materials given upon request PCR-RFLP: Maximum quantity 10uM dNTPs 2* Forward primer 2* Reverse primer 2* Taq polymerase (1U/ul) 2* Restriction enzyme X and Y 2# * Each quantity is enough for PCR of 4 samples # Each quantity is enough for restriction digestion of 3 samples ELISA: Maximum quantity 96-well ELISA plate 1 1μg/mL contaminant standard 2+ 2 water samples 2 Capture antibody 2^ Primary detection antibody 2^ Conjugated secondary antibody 2^ + Each quantity is 5μL in a PCR tube ^ Each quantity is enough for dilution in 3mL PBS/ diluent buffer Remark: – All other materials will be provided on the teaching bench in a self-service manner – Take only the quantities required for your experiment – Keep all storable materials in the storage box provided at the end of every lab session 3. Arrangement for running PAGE Each PAGE gel tank can run a maximum of 4 gels at the same time and there are 3 gel tanks provided. As the electrode assembly requires a pair of 2 gels to hold the TBE buffer inside (refer to demo video “PAGE gel casting”), it is suggested that each group finds another group and try best to run their gels together.
HI SIR. FOR LAB REPORT I FOUNF THE URL
2020 autumn BIOL S356F Biochemical and DNA Technologies Laboratory Raw data Experiment 1 – Species Identification of Meats by PCR -RFLP Below are images of PCR products of three unknown samples and digested PCR products of three reference meat samples * with 100 bp Plus DNA ladder, run on agarose gel and stained with GelRed * PCR and restriction digestion of reference meat samples were done by staffs Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 Lane 8 Lane 9 Lane 10 Lane 11 DNA Marker Negative Control PCR Product BsaJI RsaI A B C Beef Chic Pork Beef Chic Pork Reference result obtained from teaching staff s: Result obtained from group X : 2020 autumn Below are images of digested PCR products of three unknown samples with 100 bp Plus DNA ladder, run on polyacrylamide gel and stained with GelRed Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 Lane 7 DNA Marker Enzyme X Enzyme Y Sample A Sample B Sample C Sample A Sample B Sample C Reference result obtained from teaching staffs: Result obtained from group X (using PCR products from another group for digestion ): 2020 autumn Experiment 2 – Detection, Quantitation, and Identification of Target Contaminant in Water Below are tables show ing the absorbance of solution after ELISA at 450nm and 600 nm 1 2 3 4 A Blank Blank Sample 1 original Sample 1 original B Standard 1st dilution* Standard 1st dilution* Sample 1 2-fold dilution Sample 1 2-fold dilution C Standard 2nd dilution Standard 2nd dilution Sample 1 4-fold dilution Sample 1 4-fold dilution D Standard 3rd dilution Standard 3rd dilution Sample 2 original Sample 2 original E Standard 4th dilution Standard 4th dilution Sample 2 2-fold dilution Sample 2 2-fold dilution F Standard 5th dilution Standard 5th dilution Sample 2 4-fold dilution Sample 2 4-fold dilution * The 1 st dilution was prepared by adding 2μL of 1μg/mL of contaminant standard to 798μL diluent buffer ( see step 2 in part D of the procedures) Reference result obtained from teaching staffs: 450nm 1 2 3 4 A 0.092 0.099 1.067 1.019 B 1.052 0.965 0.806 0.813 C 0.799 0.761 0.605 0.618 D 0.585 0.564 0.622 0.615 E 0.456 0.443 0.487 0.467 F 0.398 0.364 0.338 0.385 Result obtained from group X : 450nm 1 2 3 4 A 0.092 0.118 0.129 0.102 B 0.080 0.100 0.109 0.110 C 0.071 0.116 0.123 0.135 D 0.072 0.103 0.101 0.113 E 0.080 0.105 0.109 0.101 F 0.096 0.099 0.104 0.102 600nm 1 2 3 4 A 0.037 0.034 0.036 0.037 B 0.034 0.034 0.035 0.035 C 0.034 0.034 0.035 0.035 D 0.034 0.034 0.034 0.034 E 0.034 0.034 0.036 0.036 F 0.034 0.039 0.034 0.034 600nm 1 2 3 4 A 0.033 0.034 0.036 0.039 B 0.037 0.035 0.035 0.035 C 0.034 0.035 0.036 0.033 D 0.039 0.038 0.036 0.037 E 0.036 0.037 0.038 0.038 F 0.038 0.037 0.037 0.038