"identification of unknown microbe"

provide a Lab report with a scientific format, and it should describe identification of microbe in the assigned data set.

Cover page (Use this specific format)

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RESEARCH REPORT

IDENTIFICATION OF UNKNOWN MICROBES

Course: Savannah State University – 3321L / 2020 Spring

Section: 1 (1 PM) or Section 2 (4 PM) (highlight one)

Group Name (type your group name): Group X

Submission option: Individual or Group (*up to 4 members per repot) (highlight one)

Authors (Student name and 915) who contribute to write this report

1. Student A / 915-XXXXX

2. Student B / 915-XXXXX

Introduction:

It is imperative to understand why one would want to differentiate between multiple types

of bacteria. In order for a person to receive proper treatment for a certain pathogen, the microbe

has to be first identified. This whole process is done for the reason that different treatments may

work effectively against one microbe, while it may prove to be ineffective against another. Also,

microbe identification is significant in processing of certain foods and development of antibiotics.

The overall purpose and aim of this investigation is to identify an unknown sample of bacteria

retrieved from the oral cavity of a group member. The following study was performed by following

a series of protocols and techniques listed in the lab manual given from Professor Takayuki Nitta

(Reference).

Materials & Methods

First, a sample was obtained from the oral cavity of a group member by using a sterile

cotton swab, and inoculated onto a T-soy agar plate. In order to maintain the freshness of the

unknown sample, the group changed the T-soy media of the bacteria by inoculating the bacteria

onto a new T-soy agar plate every 1 – 2 weeks.

The first test conducted was the Gram-staining test, —————————————.

To identify the microbe isolated from XXXX, a series of tests are completed. The summary for

the reagents, procedures used in the tests are shown in Table 1.

Table 1 Summary of methods to characterize the bacteria isolated from forehead.

Test name Purpose Reagents/

Materials

Procedures

Gram stain 1. To determine shape of
bacteria cells

2. To determine Gram
reaction of bacteria

Cristal violet

Iodine

Alcohol

Safranin

After fixation of the samples

smeared on the microslide, the

bacteria were exposed to cristal

violet, iodine, alcohol and

safranin, sequentially. The

bacteria on the slide were

observed by compound

microscope.

Colony shape To characterize shape of

bacterial colony

Dissecting

microscope

Observe bacterial colony by

microscope

e.t.c

Students need to list all methods used to identify the bacteria

Results

The unknown microbe appeared to form opaque yellow colored colonies when plated. Ultimately,

the unknown microbe was a gram-positive microbe with a staphylococcus type of morphology. It

was able to breakdown hydrogen peroxide, survive in a region of high salt concentration, coagulate

blood plasma, and also able to ferment mannitol. Table 2 below lists the tests performed along

with reagents, and also the observer’s observations and results, while Figure 1 shows the process

in which tests were completed to make the final deduction.

Table 2. Summary of results obtained through the tests
Test Observations Results/Interpretation

Gram stain Violet colored clusters of spheres Coccus-shaped and Gram-positive

Endospore stain No green was stained on slide and

grape-like clusters were seen

Cannot form endospores and the

morphology is staphylococcus

Catalase Bubbles appeared instantly Has ability to catalyze breakdown

of hydrogen peroxide

Indole test Pale yellow color Negative indole test

MSA Growth / Media became yellow Halophiles and ferment mannitol

e.t.c.

Students need to list all results obtained through the experiments

• Insert figures of your complete diagnostic key in section “Results”
Alternatively cite the figures of diagnostic key in section “Results” and cite the figures

in the main text.

(e.g. To identify the isolated microbe, we analyzed the diagnostic key. The complete diagnostic

key was inserted in this section (Fig. 1) or attached separately (Fig. 1).

III

Fig. 1. Diagnostic key for Gram Positive and Negative bacteria

(Here is one page only, but 4 pages of the completed key are required for full credits)

Discussion & Conclusion

In conclusion, it is deduced that the unkown bacteria retrieved is XXXXXXX. This

conclusion was reached due to the parallel in test results of the group’s data and that of Gloria et

al, who asserts that S. aureus is a gram positive bacterium which displays the characteristic of

clumping (Gloria et al, 2011). She then goes on to state that S. aureus is a “gram-positive

bacterium…that is also able to ferment glucose…produces catalase, nitrate, and coagulase” (Gloria

et al, 2011). According to Table 1 and Fig. 1, the tests conducted all resulted in the same findings.

Also, the unknown sample proved to be indole negative and had an inability to create spores.

(e.g. When you have additional tests to identify microbes or that were not completed)

Unfortunately, the group was not able to complete oxidation and fermentation tests because

XXXXXXXXX. In order to combat this problem in the future, the different microbiology lab

classes should be separated more carefully, and also, the group should be sure to label its materials

better.

References

1. “Microbiology (BIOL3321L) Laboratory Manual Fall 2014”. Nitta, Takayuki. (Fall 2014).
1st edition. Pgs. 1-XX. Print.

2. “Gram Stain of Staphylococcus aureus from a wound infection” Gloria D., Lewis Tomalty.
American Society for Microbiology. August 22, 2011.

Instruction for the BIOL 3321L Final Lab report (50 points)

Since we could not continue to characterize bacteria that were isolated from human or class room in classes, students must submit the final lab report with the given information. Please read this instruction carefully and submit your report by 11/20/2020 5 PM to the BIOL3321L dropbox in D2L. According to late submission policy in the syllabus, no point will be given for late submission. If you have any questions, contact the instructor ([email protected]) ASAP since to write the final report is time-consuming.

1. Instructor will assign one unknown bacterium to each group in BIOL 3321L class (including both hybrid and online students). Students can submit the report as a group or individuals. If you choose group submission, you need to clearly show who contributes to write the report. (e.g. Group A has 3 members, but two students form 1 group and one student work individually. In such case, the students who made a team must show name of both students on the provided specific format). To share the information over groups are penalized (e.g. Group A and Group B cannot use the same descriptions, tables, figures).

2. Read lab manuals 2 & 3 to find how to write a final lab report. Your lab report must have the following items,

1) Title, Name, Student ID number and Affiliation (Cover page with specific format will be provided)

2) Summary (less than 250 words)

3) Introduction

4) Materials & Methods – Show all materials and methods used to obtain the data. Students can describe everything or use a table to describe materials and methods concisely.

5) Results – Show all results with one table and describe the data.

6) Discussion & Conclusion – The section must include the 4-page diagnostic key which supports your conclusion

7) References

*You can find the rubric in the Lab manual 3. The instructor will grade the final lab report with this rubric.

*Students need to make figure(s) to show the diagnostic keys (both Gram-positive and negative keys must be submitted). Alternatively, the completed key can be scanned and submitted to the dropbox with either JPEG or PDF format. If you cannot access a scanner and need to take photos by your cell phone/camera, low resolution of the pictures/files must be avoided. The instructor will not provide any points if characters are not clear for any reasons.

Cover page (Use this specific format)

RESEARCH REPORT

IDENTIFICATION OF UNKNOWN MICROBES

Course: Savannah State University – 3321L / 2020 Spring

Section: 1 (1 PM) or Section 2 (4 PM) (highlight one)

Group Name (type your group Number):

Submission option: Individual or Group (*up to 4 members per repot) (highlight one)

Authors (Student name and 915) who contribute to write this report

Experiments & Data

Please check the assignment table and find experimental data corresponding to your group. No point will be given to the reports that chose the wrong data set. Contact the instructor ([email protected]) if you have any questions.

Group and Assignments

Group Name for Lab report	Student Name	Assigned Data Set (See below)
1A	Bennett, Kori J. Bradley, Kia K. Brewton, Deveon A. Broadwater, Dylan	Data A
1B	Brown, Kennedy D. Coleman, Robyn F. Exum, Audra L. Huynh, Huong	Data B
1C	Jackson, Jamya Jackson, Morgan D. Johnson, Mary E. Littlejohn, Jazmine A.	Data C
1D	Pellington, Toni A. Peron, Hope C. Roby, Dechelle Rosier, Aisa M.	Data D
1E	Smith, Jharnika A. Soyemi, Oluseyi B. Taylor, Branden R.	Data E
1F	Tinch, Regia L. Turner, Lakaya R. Willis, Alexandria R.	Data F
2A	Butler, Dajour T. Byrne, Briana E. Carter, Jataya C. Davis, De`jah L.	Data A
2B	Edwards, Amelda L. Frazier, Lester D. Gainor, Jantisha T. Garrett, Daja M.	Data B
2C	James, Amiya Jones, Fatima T. Lukusa-Sawelena, Bitota K. Marable, Alicia J.	Data C
2D	Mock, Dayzhia T. Odom, Lashonna T. Ohanu, Joshua C. Rasheed, Tupac A.	Data D
2E	Rivera Pagan, Charline Simmons, Sydney M. Smith, Keshawna A. Spencer, Deontis R.	Data E
2F	Tucker, Amia L. Ware, Timesha M. Weaver, Amanda D.	Data F
2G	Weston, Marnisha E. Williams, Jasmine N. Young, Tytiana	Data A

Read the information below and write a report. Students need to research materials and methods corresponding to the experiments/tests. The majority of the information for the tests is available in Lab activity notes and Lab manuals, but students need to research some tests by themselves. The table below showed observations and the report must show results that are interpreted by the students.

Data A

Isolation	Your bacterial was isolated from soil with T-soy plate
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was purple after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria Developed a deep rose color in the culture 15 minutes following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow
Lactose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Spore formation	Spores/endospores were observed after staining with malachite green
Pigment	Bacteria did not show pigments

Data B

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow
Lactose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria did not show pigments

Data C

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	bacteria did not show diffusion in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Lactose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria did not show pigments

Data D

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical turned to pink.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Lactose	After culture of bacteria in broth, the pH indicator, phenol red maintain its original color (pink).
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria show pigments (blue, yellow, red, etc)

Data E

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was rod.
Gram’s staining	The color of the bacteria was pink after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Addition of Kovac’s reagent to bacteria in the test tube formed cherry red reagent layer.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria did not grow on the plate
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Lactose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria did not show pigments.

Data F

Isolation	Your bacterial was isolated from human sample.
Shape	Under microscope, the shape of the bacteria was spherical.
Gram’s staining	The color of the bacteria was purple after Gram’s staining.
Catalase test	When H2O2 was given to the bacteria, O2 was produced.
Motility test	Motility test showed diffused bacteria in the agar media.
Oxidase test	DMPD was given to the bacteria and the chemical did not show changes.
Indole production test	Kovac’s reagent was given to the bacteria and the chemical did not show changes.
Voges-Proskauer test	The bacteria did not developed a deep rose color in the culture following the addition of Barritt’s reagent.
Mannitol salt agar	The bacteria grew on the plates and made the media yellow.
Glucose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Lactose	After culture of bacteria in broth, the pH indicator, phenol red made the media yellow.
Spore formation	Spores/endospores were not observed after staining with malachite green
Pigment	Bacteria produced yellow pigments.

“identification of unknown microbe”

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